ABSTRACT
A simple transmission electron microscope (TEM) suitable for lecture demonstrations is described. In this TEM electrons are created in a glow discharge between two parallel electrodes in air at a reduced pressure. The electrons are collimated by a small hole in the anode, focused by a solenoid that acts as an electromagnetic lens, and imaged on a thin layer of phosphor deposited inside an Erlenmeyer flask. An image of a biological sample placed between the source and the lens can be magnified about 20 times. The microscope uses inexpensive components that can be quickly assembled during a demonstration. The TEM provides a visual and memorable display that highlights phenomena such as mean-free-path, charged particle optics, electrical discharges, and cathodoluminescence.
- 1. M. Knolland and E. Ruska, “Beitrag zur geometrischen Elektronenoptik I und II (Contribution to geometrical electron optics),” Ann. Phys.0003-3804 12, 607–640 (1932); Google Scholar
M. Knolland and E. Ruska, Ann. Phys.0003-380412, 641–661 (1932). Google Scholar - 2. FEI Company, Titan TEM, www.feicompany.com/. Google Scholar
- 3. Gertrude F. Rempfer, “Electrostatic electron optics in the 1940s and today,” Microscope Soc. Am. Bull. 23, 153–158 (1993). Google Scholar
- 4. Varian, variable leak valve, model 951-5106, www.varianinc.com/. Google Scholar
- 5. E. Ruska, “The development of the electron microscope and of electron microscopy. Nobel lecture (physics),” Rev. Mod. Phys.0034-6861 https://doi.org/10.1103/RevModPhys.59.627 59, 627–638 (1987). Google ScholarCrossref
- 6.A current limiting resistor (three resistors in parallel, ) is wired in series with the power supply and the AK gap. A bleeder resistor is wired between the output of the power supply ( @ ) and ground.
- 7. A. von Engle, Ionized Gases (Oxford University Press, London, 1955). Google Scholar
- 8. See, for example, Experimental Innovations in Surface Science, edited by John T. Yates, Jr. (Springer-Verlag, New York, 1998). Google ScholarCrossref
- 9. Ted Pella, Inc., PELCO colloidal silver liquid, Part No. 16034, www.tedpella.com/. Google Scholar
- 10. Acheson Colloids Company, Aerodag G, www.achesonindustries.com/. Google Scholar
- 11. J. A. Panitz and D. G. Bear, “A procedure for increasing the contrast of biological specimens in edge-projection TEM,” J. Microsc.0022-2720 l38, 107–110 (1985). Google ScholarCrossref
Please Note: The number of views represents the full text views from December 2016 to date. Article views prior to December 2016 are not included.

